Journal: Regenerative Therapy

Article Title: 3D-printing flexible PLGA scaffold modified with bioorthogonal IGF-1 for skin regeneration

doi: 10.1016/j.reth.2026.101100

Figure Lengend Snippet: Cytocompatibility and bioactivity of different scaffolds. (A) Calcein-AM staining photos and (B) proliferation of NIH-3T3 cells on scaffold in each group. (C) The relative mRNA expression of bFGF, Col1, integrin β, and VEGF in NIH-3T3 cells on different scaffolds at 7th day. Bar = 200 μm, n = 3, ∗p < 0.05.

Article Snippet: On day 4 and 7, in order to evaluate cell adhesion, Calcein AM (Beyotime) was used to stain the cells.

Techniques: Staining, Expressing

Journal: Materials Today Bio

Article Title: Rational design of FAP-targeted sEVs delivered by microneedles for precision treatment of hypertrophic scars via ferroptosis in hypertrophic scar fibroblasts

doi: 10.1016/j.mtbio.2026.103117

Figure Lengend Snippet: In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. (N, O) Calcein-AM/PI flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Cell viability and death were assessed using a Calcein-AM/PI Cell Viability/Cytotoxicity Kit (Beyotime, China).

Techniques: In Vitro, Staining, CCK-8 Assay, Migration, Wound Healing Assay, Western Blot, Control, Immunofluorescence, Fluorescence, Flow Cytometry